| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1491 | Roseobacter denitrificans | TGCGGTGACAGCGATCAA | AAGCACTGCGCAGGTTGA | 59.66 | 60.20 | 203 | 63 |
100.00%
|
100.00%
|
| Primer 1492 | Roseobacter denitrificans | TCAACCTTGCGCACGGAT | TGGCCAGCACCTGATCAAG | 59.97 | 60.00 | 189 | 62 |
100.00%
|
100.00%
|
| Primer 1493 | Roseobacter denitrificans | TGCGCAATTCGAGCTGGA | GCATCTGCACCAGATCGGA | 60.05 | 59.86 | 185 | 62 |
100.00%
|
100.00%
|
| Primer 1494 | Roseobacter denitrificans | CAGAAACAGCTGCGCATCC | CAAGATGACCTGCACCGGA | 59.86 | 59.70 | 190 | 62 |
100.00%
|
100.00%
|
| Primer 1495 | Roseobacter denitrificans | TTACATGCCAACCGACCCC | TTGGCAGCAGTACCGCAT | 60.00 | 59.65 | 289 | 62 |
100.00%
|
100.00%
|
| Primer 1496 | Roseobacter denitrificans | TCGCTGAGCTGTTTGACCC | GGGAAAGCGCTTCGTCAAC | 60.30 | 59.79 | 225 | 62 |
100.00%
|
100.00%
|
| Primer 1497 | Roseobacter denitrificans | TTGCAGCCCAAATCCGGA | TGATCGAACTGCCGTTCCC | 59.56 | 60.08 | 278 | 62 |
100.00%
|
100.00%
|
| Primer 1498 | Roseobacter denitrificans | CTGTTCTGGGAACACGGCT | TTGCACAGGAAGCAGCCA | 59.93 | 59.80 | 272 | 61 |
100.00%
|
100.00%
|
| Primer 1499 | Roseobacter denitrificans | TGTTTCCACTGGTGCGCA | TCTTGCGCATCATCGGGT | 60.12 | 59.42 | 255 | 61 |
100.00%
|
100.00%
|
| Primer 1500 | Roseobacter denitrificans | TGGACCCTGCAAAACGCT | TGCCCTTTGGAGAACGCA | 59.81 | 59.49 | 159 | 61 |
100.00%
|
100.00%
|